Recominant Expression and Immunological Characterization of House Dust Mite Allergen Der P 1
نویسندگان
چکیده
منابع مشابه
Molecular, Structural and Immunological Characterization of Der p 18, a Chitinase-Like House Dust Mite Allergen
BACKGROUND The house dust mite (HDM) allergen Der p 18 belongs to the glycoside hydrolase family 18 chitinases. The relevance of Der p 18 for house dust mite allergic patients has only been partly investigated. OBJECTIVE To perform a detailed characterization of Der p 18 on a molecular, structural and immunological level. METHODS Der p 18 was expressed in E. coli, purified to homogeneity, t...
متن کاملExtraction of house dust mite allergen, Der p 1, from dust.
A recent article in this journal found that the type of extraction buffer did not affect measured levels of the major group one allergen (Der p 1) from the house dust mite, Dermatophagoides pteronyssinus, in household dust samples (1). This differed from a previous study where about twice the levels of Der p 1 were found in borate-buffered saline extracts, compared to ammonium bicarbonate buffe...
متن کاملCloning and expression of DNA coding for the major house dust mite allergen Der p 1 in Escherichia coli.
A cDNA clone coding for the major house dust mite allergen Der p 1 was isolated from a lambda gt 11 library. Its sequence correlates with known amino acid sequences of Der p 1 and it produces a fusion protein which reacts with rabbit anti-Der p 1 antiserum.
متن کاملβ-catenin mediates the inflammatory cytokine expression induced by the Der p 1 house dust mite allergen.
The modulations of β-catenin were analyzed during the inflammatory response induced by the Der p 1 house dust mite allergen. Der p 1 induced the dose-dependent expression of inflammatory cytokines, including interleukin (IL)-6, IL-8, monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNF-α) in THP-1 human monocytic cells. The mRNA expression levels of β-catenin were not alt...
متن کاملConversion of Der p 23, a new major house dust mite allergen, into a hypoallergenic vaccine.
Der p 23, a new, major house dust mite (HDM) allergen that is recognized by >70% of HDM-allergic patients, has high allergenic activity and, therefore, must be considered an important component for HDM-specific immunotherapy. We constructed and characterized a hypoallergenic Der p 23 vaccine for HDM immunotherapy. Three nonallergenic peptides from the C-terminal IgE epitope-containing part of D...
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ژورنال
عنوان ژورنال: Scandinavian Journal of Immunology
سال: 2008
ISSN: 0300-9475
DOI: 10.1111/j.0300-9475.2004.01423ag.x